Reporter

Part:BBa_K3267001

Designed by: Yuma Kanazawa   Group: iGEM19_NYU_Shanghai   (2019-10-15)
Revision as of 22:08, 21 October 2019 by Yumakk (Talk | contribs)


Quorum-Sensing Receiver Construct

Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1661


[edit]
Categories
Parameters
None