Regulatory

Part:BBa_K135000:Experience

Designed by: Oliver Purcell   Group: iGEM08_BCCS-Bristol   (2008-10-20)
Revision as of 15:50, 20 October 2008 by OliverP (Talk | contribs) (→‎Applications of BBa_K135000)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K135000

We have used this part, fused upstream of BBa_E0840 (BBa_K135001), and have shown GFP activation in reponse to adhesion on glass slides. The conclusion that this is activated in response to adhesion follows from Ottos and Silhavys work (see main page description for reference), in which they demonstrated activation of a pCpxR-LacZ fusion in response to adhesion to glass microspheres. Filamentous bacteria fluoresced significantly, while it was hard to ascertain whether smaller bacteria were fluorescing. Due to time constraints, a better characterisation of the efficiency of the promoter could not be performed.

User Reviews

UNIQ56cb022e2088a5cd-partinfo-00000000-QINU UNIQ56cb022e2088a5cd-partinfo-00000001-QINU