Generator

Part:BBa_K1741014:Experience

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 16:47, 21 October 2019 by Goktugattar (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1741014

Bilkent-UNAMBG 2019 iGEM Team

We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.


T--Bilkent-UNAMBG--eMPTYod.png


Figure 1:BL21 and DH5a(MG1655) OD Measurements


T--Bilkent-UNAMBG--empty.png


Figure 2: BL21 and DH5a(MG1655) RFU/OD Measurements


T--Bilkent-UNAMBG--PZE.png


Figure 3: pZe proD sfGFP RFU/OD Measurements

20191021164231%21T--Bilkent-UNAMBG--pZAProDsfGFP.png


Figure 4: pZa proD sfGFP RFU/OD Measurements


T--Bilkent-UNAMBG--PZS.png


Figure 5: pZs proD sfGFP RFU/OD Measurements


T--Bilkent-UNAMBG-PZSOD.png


Figure 6: pZe proD sfGFP OD Measurements


T--Bilkent-UNAMBG-PZAOD.png


Figure 7: pZa proD sfGFP OD Measurements


T--Bilkent-UNAMBG-PZEOD.png


Figure 8: pZs proD sfGFP OD Measurements

User Reviews

UNIQ60308ff0de59e423-partinfo-00000000-QINU UNIQ60308ff0de59e423-partinfo-00000001-QINU