Coding

Part:BBa_K3257047

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-16)
Revision as of 16:05, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Truncated mCherry

This part is a truncated mCherry and it only contains amino acid residues from the1st to the 158th of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None