Composite

Part:BBa_K3286008:Design

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 21:12, 21 October 2019 by AlexNiederau (Talk | contribs) (→‎References)


IPTG inducible dCas9 expression module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References

  1. Larson, M. H., Gilbert, L. A., Wang, X., Lim, W. A., Weissman, J. S., & Qi, L. S. (2013). CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature protocols, 8(11), 2180.