Composite

Part:BBa_K2916023

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-26)
Revision as of 22:42, 26 September 2019 by Konstantinos (Talk | contribs)


Expression of IleRS in E.coli

This part is used for expression of IleRS needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2996
    Illegal NotI site found at 2874
    Illegal NotI site found at 2936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 937
    Illegal BamHI site found at 1440
    Illegal XhoI site found at 2945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 2015
    Illegal AgeI site found at 2474
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1559


[edit]
Categories
Parameters
None