DNA

Part:BBa_K3060005

Designed by: Jingyi Zhu   Group: iGEM19_DUT_China_A   (2019-10-16)
Revision as of 20:18, 16 October 2019 by Registry (Talk | contribs)

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RCA&MCA Primer3 for DNA hydrogel

This part is a ssDNA, cooperating with BBa_K3060002-BBa_K3060007 to form the DNA hydrogel. This part can not only help the linear template to form the circular template, but also complimentary with circular template. The conjugate can be recognized by phi29 DNA polymerase and triggers the rolling circle amplification (RCA, or R). After the RCA completed, adding this part and primer2 (BBa_K3060004) to start the multi-primed chain amplification (MCA, or M).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None