Regulatory

Part:BBa_K3030012

Designed by: Xiaomeng Liu   Group: iGEM19_XJTLU-CHINA   (2019-09-13)
Revision as of 12:27, 14 October 2019 by LiuXiaomeng (Talk | contribs)


MS2-responsive ribozyme switch (Off to On) + PP7 coat protein

This part is a combination of MS2 ribozyme switch and PP7 coat protein to manipulate regulation process with another part automatically in the mammalian cells. We built up a three plasmids circuit which aimed to subsequently regulate expression level of gene(s) connected by 2A peptide with regulating protein (MS2/PP7).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None