Part:BBa_K3286000:Design
AcrIIA4
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
non-codon optimised
Source
non-codon optimised version retrieved from NCBI
References
Rauch, B. J., Silvis, M. R., Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of CRISPR-Cas9 with bacteriophage proteins. Cell, 168(1-2), 150-158.
Kim, I., Jeong, M., Ka, D., Han, M., Kim, N. K., Bae, E., & Suh, J. Y. (2018). Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor. Scientific reports, 8(1), 3883.
Dong, D., Guo, M., Wang, S., Zhu, Y., Wang, S., Xiong, Z., ... & Huang, Z. (2017). Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein. Nature, 546(7658), 436.
Yang, H., & Patel, D. J. (2017). Inhibition mechanism of an anti-CRISPR suppressor AcrIIA4 targeting SpyCas9. Molecular cell, 67(1), 117-127.
Shin, J., Jiang, F., Liu, J. J., Bray, N. L., Rauch, B. J., Baik, S. H., ... & Doudna, J. A. (2017). Disabling Cas9 by an anti-CRISPR DNA mimic. Science advances, 3(7), e1701620.