Coding
mClover3

Part:BBa_K3183010

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-14)
Revision as of 19:38, 21 October 2019 by Dschramm (Talk | contribs) (→‎Parts characterized by Oxford iGEM 2019)


mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Fluorescence wavelengths


Bajar et al report the following excitation and emission data for mClover3 -

  • Excitation max - 506nm
  • Emission max - 518nm




Figure 1: mClover3 spectrum



Parts characterized by Oxford iGEM 2019

Figure 2: mClover3 Log Standard curve
Figure 2: - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. Error bars represent Standard error of the mean. n=3



Characterisation

This part was characterised in the composite part BBa_K3183028, and BBa_K3183101.

Use by Team Oxford 2019

This part was used in the following composite parts: BBa_K3183028, BBa_K3183300, BBa_K3183104, BBa_K3183203, BBa_K3183101, and BBa_K3183104.

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