Part:BBa_K538101

RBS + Cpn60

K538101

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. It was used as a construction intermediate, to create the following composite parts:

• BBa_K538201
• BBa_K538210
• BBa_K538211
• BBa_K538301
• BBa_K538310
• BBa_K538311

For more information about this brick's biology, refer to the page of its coding region: BBa_K538001

Results

As part of charecterization, the iGEM IISER Bhopal 2019 team performed growth curves at different temperatures in order to study the part which was also a part of our project.

Cpn 60 was successfully digested from the parent backbone pSB1C3, which was confirmed on gel via size confirmation and sequencing exercises.

As this part had already been submitted by the 2011 Amsterdam team, we decided to further characterize it (as a part of the Bronze medal criteria) and check how its induction affected cell growth. The results of the growth analysis may be seen in the graphs provided below. The temperatures chosen were 37°C (as a model for optimal conditions) and 18°C (as a model for suboptimal conditions). While we ideally would have liked to achieve even lower temperatures (as our model clearly predicts that TF could survive near 0°C-like conditions), we were most unfortunately limited by the available infrastructural facilities.</p>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 888
  Illegal BamHI site found at 954
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]