Part:BBa_K3225006

EcbR, transcriptional factor

Quorum sensing is a well-known cell-cell communication system to many bacteria as well as the plant pathogenic bacteria E.carotovora. The transcriptional factors ExpRs play the same role as LuxR in the E.carotovora quorum sensing systems. ExpRs are the receptors of OHHL (a quorum sensing signal of E. carotovora). After binding to OHHLs, ExpRs can positively or negatively regulate the protein expression. ExpR153(BBa K3225002), ExpR71(BBa K3225005), and EcbR(BBa K3225006) are the OHHL receptors in three E.carotovora subspecies. And all of the three receptors can bind a promoter pExp and regulate the downstream protein expression. Here we characterized the binding and induction features of three receptors of E. carotovora. The three receptors (ExpR153, ExpR71, and EcbR) were constitutive expressed under a constitutive promoter down streamed with the pExp regulating reporters (GFP).

1)J23101- ExpR153 -pExp-GFP

2)J23101- Exp71-pExp-GFP

3)J23101- EcbR - pExp-GFP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 717
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 249