Reporter

Part:BBa_K3026001

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)
Revision as of 17:38, 8 September 2019 by Registry (Talk | contribs)

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ToeHold_RNA_AmpR

ToeHold switch targeting mRNA sequence of pBluescript ampicillin resistance cassette. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the mRNA sequence of the ampicillin resistance cassette. When this ToeHold is in presence of this mRNA sequence, the sfGFP will be produced. This system allows for detection of DNA fragments or plasmids expressing this cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


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Categories
Parameters
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