RNA

Part:BBa_K3081111

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 11:30, 21 October 2019 by Lelezhang (Talk | contribs)


a 20bp sequence targeting the R1(bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R1- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058


T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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