Translational_Unit

Part:BBa_K3050000

Designed by: Kita Mizuki   Group: iGEM19_Gunma   (2019-10-15)
Revision as of 20:06, 15 October 2019 by Registry (Talk | contribs)

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pT7+RBS+mag-2+GFP+double_terminator

Magainin-2 expressed inside E.coli combines to cell membrane and damages it.In our project, we used it to weaken the cell membrane to make it easier to lyse after cell wall is broken by T7 lysozyme in BL21(DE3)pLysS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 190


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Categories
Parameters
None