Coding

Part:BBa_K3258018

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)
Revision as of 23:01, 16 October 2019 by Registry (Talk | contribs)

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Q-beta EFTu-EFTs fusion protein

Qbeta replicase beta subunit fused to EF-Tu and EF-Ts, both proteins required for the full replicase to assemble properly. It was shown that this fusion does not require ribosomal protein S1 to be active.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 535
    Illegal BamHI site found at 3399
    Illegal BamHI site found at 3542
    Illegal XhoI site found at 3073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 535
    Illegal AgeI site found at 688
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 1537
    Illegal AgeI site found at 3555
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None