Reporter

Part:BBa_K3179004

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 00:54, 20 October 2019 by Maggie-WZE (Talk | contribs)


2Kt-E1A

This part is constructed by adding a 2X Kturn sequence before the E1A gene of Ad5. Due to the existence of 2X Kturn, the translation of E1A is regulated by L7Ae. E1A is an early gene of adenovirus and is essential in the commence of viral replication. This part has an intron which also exists in E1A of the wild adenovirus. Using the splicing system in eukaryotes can make it function as normal. In our design, 2kt-E1A is used as a response part to initiate viral gene expression and lyse target cells.

We integrate 2kt-E1A with miR885-5p-target and miR663b-target so that while miRNA885-5p and miRNA663b are absent in the cell, E1A can be translated and initiate viral gene expression to lyse target cells.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 859
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 859
    Illegal NgoMIV site found at 333
    Illegal AgeI site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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