Generator

Part:BBa_K602008

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-05)
Revision as of 18:24, 21 October 2019 by Youma (Talk | contribs)

D. radiodurans RecA expression device

D. radiodurans RecA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses RecA. Should increase DNA damage repair capability and radioresistance of host E. coli.

Please see Experience page for characterization details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Botchan_Lab_Tokyo 2019 characterization

[edit]
Categories
Parameters
None