Part:BBa_J10053:Experience
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how you used this part and how it worked out.
Applications of BBa_J10053
Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.
User Reviews
Lambert_GA 2019 Characterization
Characterization of Medium Promoter (BBa_J22016) and Weak RBS (BBa_B0031)
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
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