Part:BBa_K2832003

PpspA - Inducible Promoter

Promoter pspAp aids the pspABCE "phage shock protein" operon system in response to a variety of extracellular stresses by upregulating the pspA gene. The PspA protein specifically responds to a stress-induced leaky membrane and a damaged proton motive force (PMF). These would in turn result in the While a leaky membrane may result in the influx of protons that cannot be checked by the damaged PMF, which expels protons under normal conditions, PspA can respond to the stress by expelling the protons. PspA suppresses itself under normal conditions by inhibiting its transcriptional activator, PspF. However, under stress, PspF is released by PspB and PspC and can activate PspA by binding to the pspAp promoter. For our system, the binding site of the pspAp sequence for PspF was selected within -142 b.p. before the transcription start site for mRFP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 115
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 13
  
• 1000
  COMPATIBLE WITH RFC[1000]