Composite

Part:BBa_K2974500:Experience

Designed by: Janet Standeven, Abby Bell, Ashtyn Cauffman, Monica Cho   Group: iGEM19_Lambert_GA   (2019-10-16)
Revision as of 12:59, 21 October 2019 by Monicacho123 (Talk | contribs)


Medium Promoter (BBa_J23106) Toehold GFP
Sequencing of the transformation of part BBa_K2974500 confirmed the toehold construct’s function when it was aligned with the desired toehold sequence. In addition, the results for the toehold was confirmed through a dual plasmid transformation in BL21 E.coli cells. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the dual plasmid transformation with part BBa_K2974700, a toehold construct with a strong promoter (BBa_J23100). The biobricked toehold's (BBa_K2974500) function has been validated through experimentation and sequencing.

[image] [caption]
[image] [caption]

Sequence Analysis

[image] [caption]

Applications of BBa_K2974500

User Reviews

UNIQc116c85675bb9b28-partinfo-00000002-QINU UNIQc116c85675bb9b28-partinfo-00000003-QINU