Part:BBa_K2992005

ctfB from C. Botulinum

ctfB encodes the B sub-unit of Butyrate-acetoacetate CoA-transferase complex.

Usage and Biology

The CtfAB complex is central to to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. the CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

This is a basic part utilised in our composite part characterised in our acetone producing reporters:BBa_K2992028, BBa_K2992029, BBa_K2992030, BBa_K2992034, BBa_K2992035, BBa_K2992036. This part was used as a promoter which functions in both the Gram-negative E. coli and the Gram-positive Clostridium sporogenens. More information can be found on our Results Page.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Vital, M., Howe, A. and Tiedje, J. (2014). Revealing the Bacterial Butyrate Synthesis Pathways by Analyzing (Meta)genomic Data. mBio, 5(2).