Coding
Cah

Part:BBa_K2984079

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)
Revision as of 17:40, 21 October 2019 by Madparry (Talk | contribs)


Carbonicanhydrase


Our carbonic anhydrase constructs are supposed to enhance chlamydomanas reinhardtii´s capability to use carbonic acid as a carbon source. The Aminoacid-sequence of our carbonic anhydrase was obtained via uniprot. The enzyme catalyzes the reaction depicted below. Chararacterization of this part may be possible by using media with different concentrations of carbonic acid in a algae growth measurement. Sadly we didn´t have time to plan and carry out any transformations or characterizations with this part. This sequence also contains two rbcS2i1 introns inserted as suggest by Baier et al, 2018.




Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 241
    Illegal XhoI site found at 427
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
    Illegal NgoMIV site found at 364
    Illegal NgoMIV site found at 1251
    Illegal AgeI site found at 1192
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B3-B4 MoClo overhangs can be found here.


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