Part:BBa_K2915150

IPTG inducible promoter (T7) with RBS AFP-lu1 6-His-Tag with TEV site

The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.The AFP protein was produced and purified because of the presence of a 6His-Tag in the N-terminal of the protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL1 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.