Regulatory

Part:BBa_K3100001

Designed by: Zheng Yiyuan   Group: iGEM19_SCUT_China   (2019-08-31)
Revision as of 17:15, 21 October 2019 by 201730851287zyy (Talk | contribs)


T7 Promoter T7-1
PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7. From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it: https://2019.igem.org/Team:SCUT_China/Design

T - SCUT China- T7 promoter 1-48.jpeg
Figure. The strength of PT7 1-48

References:

Ghodasara A, Voigt CA. Balancing gene expression without library construction via a reusable sRNA pool. Nucleic Acids Res. 2017;45(13):8116–8127. doi:10.1093/nar/gkx530

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None