Part:BBa_K3225015

J23101-LuxR-pLux-LacZ

This gene circuit is designed to detect N-(3-oxohexanoyl)-L-homoserine lactone (OHHL ，quorum sensing signal molecular of E.carotovora). It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drove by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drove by the binding promoter of LuxR(pLux, BBa_R0062).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Results

We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).

Figure 1. The detection limit and detection time of quorum sensing based testing strips.

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