Regulatory
TEF1p

Part:BBa_K2117000

Designed by: Isabella Loft   Group: iGEM16_DTU-Denmark   (2016-10-14)
Revision as of 16:50, 17 October 2016 by SidselClemmensen (Talk | contribs)


Constitutive TEF1 promoter native to Yarrowia lipolytica

The translation elongation factor-1α (TEF1) promoter is a strong constitutive promoter. Native for the oleaginous yeast Yarrowia lipolytica.

This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


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Categories
Parameters
None