Coding

Part:BBa_K2950002

Designed by: Shuxin Xiao   Group: iGEM19_BNDS_China   (2019-10-16)
Revision as of 05:46, 16 October 2019 by Registry (Talk | contribs)

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Toluene dioxygenase(TodC1C2)

Toluene dioxygenase is an ISP protein identified from Pseudomonas putida which contains alpha subunit(TodC1) and beta subunit(TodC2). TodC1 carries the oxidation reaction of toluene under the presence of Tod C2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
    Illegal BglII site found at 714
    Illegal BglII site found at 729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1527
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 1384
    Illegal NgoMIV site found at 1905
    Illegal NgoMIV site found at 1947
    Illegal AgeI site found at 1511
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None