Coding

Part:BBa_K3031018

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)
Revision as of 00:40, 16 October 2019 by Davystar (Talk | contribs)


OxyR mutated at residues 147 & 203

OxyR Transcription factor protein. OxyR reacts with H2O2 resulting in the oxidation of a reactive cysteine (Cys-199) formation of a intermolecular disulfide bond and thus a conformation change in the OxyR protein. The activated OxyR then binds to certain promoter regions to regulate the expression of downstream genes.

This is an improvement to the part BBa_K1104200 submitted by National Yang Ming University iGEM Team 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 673


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Parameters
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