Composite

Part:BBa_K3126022

Designed by: Qianxin Li   Group: iGEM19_HBUT-China   (2019-10-16)
Revision as of 08:04, 20 October 2019 by Lqs991122 (Talk | contribs)


nixA-TgMTP1t2-KanR

We connected TgMTP1t2 and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part. We also compared its function with those two functional genes working separately (Part:BBa_K3126020 Part:BBa_K3126019).


Usage and Biology

Here is the curve comparing with Saccharomyces cerevisiae expressing Part:BBa_K3126020 and Part:BBa_K3126019. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with BBa_K3126020 and BBa_K3126019

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2574
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4048
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None