Composite

Part:BBa_K3110018:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 16:27, 14 October 2019 by Registry (Talk | contribs)

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Medium Promoter Weak RBS lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1987
    Illegal AgeI site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1424


Design Notes

lldD was codon optimized to meet synthesis requirements and to remove the illegal EcoRI site present in the genomic lldD.



Source

lldD was Synthesized from Twist Bioscience and was joined to lldR using SOEing (Spliced Overlap Extension) PCR. This was further joined to Promoter+RBS and Terminator using SOEing (Spliced Overlap Extension) PCR.


References