Coding
Mlut11700

Part:BBa_K3038004:Design

Designed by: Anas CANTEAU   Group: iGEM19_Poitiers   (2019-10-15)
Revision as of 15:21, 21 October 2019 by Acante (Talk | contribs)


Acyl CoA oxydase : Mlut11700_N-ter C-myc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1682
    Illegal XhoI site found at 1925
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1816
    Illegal BsaI.rc site found at 2047
    Illegal SapI site found at 172


Design Notes

C MYC Tags has been added on the Nterminal part of the sequence.

The sequence is flanked with the sequence of EcoRI and XbaI on the 5'end and the SpeI and PstI on the 3' end.

T--Poitiers--TDesignMlut.png
Design of the Mlut_11700 gene with the Geneious Software.
The design show restriction enzymes like EcoRI, XbaI, SpeI and PstI. The gene includes pBAD promoter and its terminater. Finally C-myc tag is present in N-term.

The RFC10 standard was followed.

The GC content has been optimized for the synthesis by IDT

Source

Sequence synthezized by IDT


References

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610