Reporter

Part:BBa_K3170999

Designed by: Haodong Yan   Group: iGEM19_LZU-CHINA   (2019-10-16)
Revision as of 12:14, 16 October 2019 by Registry (Talk | contribs)

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FRET system for detecting TEVp activity

We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None