Reporter
Part:BBa_K3170999
Designed by: Haodong Yan Group: iGEM19_LZU-CHINA (2019-10-16)
FRET system for detecting TEVp activity
We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |