Coding
mGFP5

Part:BBa_K3183011

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-14)
Revision as of 14:46, 21 October 2019 by Dschramm (Talk | contribs)


Modified Green Fluorescent Protein, Codon Optimized for L. reuteri

mGFP5 is a derivative of GFP with enhanced folding capabilities and removal of a distant intron site.1


Usage and Biology

Mutant of Aquora victoria green fluorescent protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Characterization

The part was characterized in the context of BBa_K3183100.

References

1. Haseloff, James. “Chapter 9: GFP Variants for Multispectral Imaging of Living Cells.” Methods in Cell Biology Green Fluorescent Proteins, 1998, pp. 139–151., doi:10.1016/s0091-679x(08)61953-6.

[edit]
Categories
Parameters
None