Composite

Part:BBa_K3202069

Designed by: Mingyang Xu   Group: iGEM19_BHSF_ND   (2019-10-16)
Revision as of 14:46, 21 October 2019 by MichaelNi Petrel (Talk | contribs)


AraC-pBAD-LacI Bistable System Kit

This part is designed as a tool kit for any study that demands a sequence inserted within the bistable system. BsaI sites are inserted between T500 and RBS.

See more about our recombinase flipping module below.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2393
    Illegal SpeI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2393
    Illegal SpeI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2393
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2393
    Illegal SpeI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2393
    Illegal SpeI site found at 2323
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2380
    Illegal BsaI.rc site found at 2370
    Illegal SapI site found at 961


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Categories
Parameters
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