Composite

Part:BBa_K3126018

Designed by: Qianxin Li   Group: iGEM19_HBUT-China   (2019-10-16)
Revision as of 07:44, 20 October 2019 by Lqs991122 (Talk | contribs)


hexa-His-KanR

Hexa- His gene with TEF1 promoter and CYC1 terminator, using KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part.


Usage and Biology

Here is the curve comparing with original Saccharomyces cerevisiae. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with original yeast

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2842
    Illegal AgeI site found at 1049
    Illegal AgeI site found at 1310
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None