Regulatory

Part:BBa_K2913022

Designed by: Pengchao Wang   Group: iGEM19_NEFU_China   (2019-10-20)
Revision as of 12:24, 21 October 2019 by Yingying-Chen (Talk | contribs)


P-BAD

The pBAD expression system allows tightly controlled, titratable expression of your protein through the regulation of specific carbon sources such as glucose, glycerol, and arabinose. pBAD is ideal for expressing toxic proteins and optimizing protein solubility in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82


[edit]
Categories
Parameters
None