Regulatory
Part:BBa_K091106
Designed by: Andrew Gordon Group: iGEM08_Davidson-Missouri_Western (2008-06-19)
LsrA/cI hybrid promoter
This hybrid promoter uses the LsrA promoter that is capable of being repressed by LsrR - induction can occur with phospho-AI-2. Binding sites for cI repressor also occur. As a result the promoter is off in the absence of AI-2 and on in the presence of AI-2, but always off in the presence of cI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//regulation/multiple
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//regulation/multiple
control | |
direction | Forward |
negative_regulators | 2 |
o_h | |
o_l | |
positive_regulators |