Composite
Part:BBa_K3320019
Designed by: Joseana Mendes Comodaro Group: iGEM19_Amazonas-Brazil (2019-10-20)
Lactate Repressor Unit
This is a part from our AND Gate, called lactate repressor unit. In the presence of lactate, the PhlF Repressor protein is expressed, repressing the PhlF promoter with Tet repressor, allowing the production of GFPmut3 + HlyA
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 965
Illegal NheI site found at 988 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3284
[edit]
Categories
Parameters
None |