Intermediate
Part:BBa_K2974310:Design
Designed by: Janet Standeven, Megan Hong, Courtney Taing, Julia Chang, Shanthi Hegde, Sri Pinnamareddy Group: iGEM19_Lambert_GA (2019-10-16)
C. elegans Toehold Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 175
Illegal NotI site found at 6
Illegal NotI site found at 460 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 96
Design Notes
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Source
The lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic) is native to the genome of C. elegans.