Part:BBa_K3100012

toehold switch C

Usage and Biology:

The toehold switches are classes of riboregulators that are de-novo-designed based on influence of secondary structure on mRNA translation and knowledge of RNA thermodynamics and kinetics. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon. The trigger RNA can specifically bind to a single-stranded toehold sequence at the 5’ end of the hairpin module, and expose the RBS and start codon by a branch migration process that depends on the complement of an extended single-stranded region and the hairpin, thereby initiating translation.

Fig, 1: Toehold Switch and Trigger RNA

The new classes of regulatory components offer wide dynamic range and low system crosstalk. We selected four toehold switches with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.

Characterization:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]