![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2926052
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Revision as of 12:01, 21 October 2019 by Jopgenoorth (Talk | contribs)
Fusion protein of mCherry and M13 bacteriophage protein pVIII
The major coat protein pVIII of the M13 bacteriophage was fused c-terminally to the fluorescence reporter protein mCherry.
Usage and Biology
This part was used during Troygenics Assembly to visualize the produced M13 bacteriophage-derived Troygenics via fluorescence measurement (Fig. 1).
![](https://2019.igem.org/wiki/images/3/31/T--Bielefeld-CeBiTec--Fluorescence_norm..png)
We compared the emission spectrum of our Troygenics with the emission spectrum of mCherry, because the fluorescence marker cloned on the Troygenics is mCherry, too. We have seen a difference in the emission peak of about 20 nm. This is probably because the mCherry on our Troygenics is fused to the whole Troygenic. Because of this, there might be a different folding resulting in a shift of the emission spectrum.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |