Coding

Part:BBa_K3098009

Designed by: Chenyi HE   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 10:27, 1 October 2019 by Registry (Talk | contribs)

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GST+PgD5+Avitag

This part is the one we are hoping to use in the production of the functional PgD5. In this part, GST tag helps the dissolution and purification of the peptide while Avitag provides a site for biotinylation as a linkage to the dCBM3a and BC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None