RNA

Part:BBa_K3081113

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 14:58, 19 October 2019 by Xiaoying (Talk | contribs)


a 20bp sequence targeting the DNA unwinding element on E.coli oriC

The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see BBa_K3081058


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None