Coding

Part:BBa_K3098008

Designed by: Chenyi HE   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 11:25, 21 October 2019 by Chenyihe (Talk | contribs)


GST+PgD5

In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, it can help the peptide dissolve in cytoplasm to help the expression of the peptide.

<During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease will be added to cut the GST tag from the target protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None