Coding

Part:BBa_K3098008

Designed by: Chenyi HE   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 07:10, 1 October 2019 by Registry (Talk | contribs)

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GST+PgD5

In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, GST tag can help the peptide dissolve in cytoplasm to help the expression of the peptide. During purification, GST can bind with the column , after adding the specific enzyme, GST tag can be cut to free the target peptide PgD5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None