RNA

Part:BBa_K3171182

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-20)
Revision as of 18:11, 20 October 2019 by Yashikavenkatesh (Talk | contribs)


HisD gRNA 3

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts Part:BBa_K3171177, Part:BBa_K3171178, Part:BBa_K3171179 in order to decide on the correct homologous part required for knockout. 3 gRNA target sites Part:BBa_K3171180, Part:BBa_K3171181, Part:BBa_K3171182 were selected for knock out to determine the exact region for knockout.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None