Composite

Part:BBa_K3110006

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 19:32, 18 October 2019 by Meghamariajacob (Talk | contribs)


Strong Promoter Weak RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a weak RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower and higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 638


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Categories
Parameters
None