Composite

Part:BBa_K3021004

Designed by: Jeremy Hu   Group: iGEM19_PuiChing_Macau   (2019-10-14)
Revision as of 00:24, 15 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


NSP4-Laccase1326-HisTag

This is a new composite part designed to allow Laccase secretion from our engineered E.coli strain through embedded the new secretion peptide, novel signal peptide 4 (NSP4). Laccase is an important enzyme, which would degrade different chemicals. The Laccase sequence in this part is based on the laccase1326 cDNA sequence from previous study, which was shown to have great tolerant to different harsh conditions commonly found in polluted water. Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production. NSP4 is utilizing part of the Sec-dependent (Sec) secretion pathway, the general secretion E. coli route.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
    Illegal BamHI site found at 1526
    Illegal XhoI site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862


[edit]
Categories
Parameters
None