Regulatory

Part:BBa_K3170997

Designed by: Haodong Yan   Group: iGEM19_LZU-CHINA   (2019-10-16)
Revision as of 10:13, 16 October 2019 by Registry (Talk | contribs)

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HRE(Hypoxia response elements)

In solid tuumors, rapid proliferation of tumor cells leads to increased oxygen consumption of tumor cells, which in turn leads to hypoxia microenviroment. Hypoxia is one of the basic characteristics of tumor microenvironment, playing an important role in the proliferation, invasion and metastasis of tumor cells. In our experiments, we transfect 293T cell with HRE-miniCMV and when it senses the condition of hypoxia in tumor microenvironment, HIF-1(hypoxia inducible factor 1) is produced, and it can bind to HRE(hypoxia response elements), then activating the minimized CMV promoter. Through this mechanism, we can switch the interest gene specifically in tumor microenvironment. In order to enhance its induction effect,we designed six HRE repeats components.After pHRE, miniCMV was added to transcribe the downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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